Header list of 2d93.pdb file
Complete list - r 9 2 Bytes
HEADER SIGNALING PROTEIN 08-DEC-05 2D93 TITLE SOLUTION STRUCTURE OF THE CNMP_BINDING DOMAIN OF HUMAN RAP GUANINE TITLE 2 NUCLEOTIDE EXCHANGE FACTOR 6 COMPND MOL_ID: 1; COMPND 2 MOLECULE: RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 6; COMPND 3 CHAIN: A; COMPND 4 FRAGMENT: CNMP_BINDING DOMAIN; COMPND 5 SYNONYM: PDZ DOMAIN CONTAINING GUANINE NUCLEOTIDE EXCHANGE FACTOR 2, COMPND 6 PDZ-GEF2, RA-GEF-2; COMPND 7 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 3 ORGANISM_COMMON: HUMAN; SOURCE 4 ORGANISM_TAXID: 9606; SOURCE 5 GENE: KAZUSA CDNA HA02566; SOURCE 6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 7 EXPRESSION_SYSTEM_PLASMID: P050404-16; SOURCE 8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS KEYWDS CNMP_BINDING DOMAIN, RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 6, PDZ KEYWDS 2 DOMAIN CONTAINING GUANINE NUCLEOTIDE EXCHANGE FACTOR 2, PDZ-GEF2, KEYWDS 3 RA-GEF-2, RAPGEF6, STRUCTURAL GENOMICS, NPPSFA, NATIONAL PROJECT ON KEYWDS 4 PROTEIN STRUCTURAL AND FUNCTIONAL ANALYSES, RIKEN STRUCTURAL KEYWDS 5 GENOMICS/PROTEOMICS INITIATIVE, RSGI, SIGNALING PROTEIN EXPDTA SOLUTION NMR NUMMDL 20 AUTHOR K.INOUE,Y.MUTO,F.HAYASHI,S.YOKOYAMA,RIKEN STRUCTURAL AUTHOR 2 GENOMICS/PROTEOMICS INITIATIVE (RSGI) REVDAT 3 09-MAR-22 2D93 1 REMARK SEQADV REVDAT 2 24-FEB-09 2D93 1 VERSN REVDAT 1 08-JUN-06 2D93 0 JRNL AUTH K.INOUE,Y.MUTO,F.HAYASHI,S.YOKOYAMA JRNL TITL SOLUTION STRUCTURE OF THE CNMP_BINDING DOMAIN OF HUMAN RAP JRNL TITL 2 GUANINE NUCLEOTIDE EXCHANGE FACTOR 6 JRNL REF TO BE PUBLISHED JRNL REFN REMARK 2 REMARK 2 RESOLUTION. NOT APPLICABLE. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 PROGRAM : XWINNMR 3.5, CYANA 2.0.17 REMARK 3 AUTHORS : GUNTERT, P. (CYANA) REMARK 3 REMARK 3 OTHER REFINEMENT REMARKS: NULL REMARK 4 REMARK 4 2D93 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 13-DEC-05. REMARK 100 THE DEPOSITION ID IS D_1000025140. REMARK 210 REMARK 210 EXPERIMENTAL DETAILS REMARK 210 EXPERIMENT TYPE : NMR REMARK 210 TEMPERATURE (KELVIN) : 298 REMARK 210 PH : 7.0 REMARK 210 IONIC STRENGTH : 120MM REMARK 210 PRESSURE : AMBIENT REMARK 210 SAMPLE CONTENTS : 1.06MM U-15N, 13C-LABELED REMARK 210 PROTEIN; 20MM D-TRIS-HCL; 100MM REMARK 210 NACL; 1MM D-DTT; 0.02% NAN3; 10% REMARK 210 D2O REMARK 210 REMARK 210 NMR EXPERIMENTS CONDUCTED : 3D_15N-SEPARATED_NOESY; 3D_13C REMARK 210 -SEPARATED_NOESY REMARK 210 SPECTROMETER FIELD STRENGTH : 800 MHZ REMARK 210 SPECTROMETER MODEL : AVANCE REMARK 210 SPECTROMETER MANUFACTURER : BRUKER REMARK 210 REMARK 210 STRUCTURE DETERMINATION. REMARK 210 SOFTWARE USED : NMRPIPE 20031121, NMRVIEW 5.0.4, REMARK 210 KUJIRA 0.93191, CYANA 2.0.17 REMARK 210 METHOD USED : TORSION ANGLE DYNAMICS REMARK 210 REMARK 210 CONFORMERS, NUMBER CALCULATED : 100 REMARK 210 CONFORMERS, NUMBER SUBMITTED : 20 REMARK 210 CONFORMERS, SELECTION CRITERIA : TARGET FUNCTION, STRUCTURES WITH REMARK 210 THE LOWEST ENERGY, STRUCTURES REMARK 210 WITH THE LEAST RESTRAINT REMARK 210 VIOLATIONS REMARK 210 REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1 REMARK 210 REMARK 210 REMARK: NULL REMARK 215 REMARK 215 NMR STUDY REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON REMARK 215 THESE RECORDS ARE MEANINGLESS. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC REMARK 350 APPLY THE FOLLOWING TO CHAINS: A REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2) REMARK 500 REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI- REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400 REMARK 500 REMARK 500 M RES CSSEQI PSI PHI REMARK 500 1 ALA A 47 -178.59 -50.05 REMARK 500 1 GLU A 57 95.59 -63.34 REMARK 500 1 MET A 83 120.49 -35.32 REMARK 500 1 PRO A 91 2.92 -69.83 REMARK 500 1 ILE A 122 -63.82 -103.37 REMARK 500 1 HIS A 125 65.99 -111.49 REMARK 500 2 GLU A 57 104.98 -56.73 REMARK 500 2 MET A 83 119.69 -39.30 REMARK 500 2 PRO A 91 2.81 -69.72 REMARK 500 2 ASP A 94 171.08 -52.70 REMARK 500 2 ILE A 122 -71.63 -96.56 REMARK 500 2 PRO A 131 88.80 -69.76 REMARK 500 3 ASP A 8 -54.96 -131.25 REMARK 500 3 ASP A 9 43.18 -99.06 REMARK 500 3 GLU A 57 92.63 -54.05 REMARK 500 3 MET A 83 118.62 -37.68 REMARK 500 3 ASN A 85 -179.39 -61.41 REMARK 500 3 PRO A 91 2.65 -69.74 REMARK 500 3 ILE A 122 -54.49 -122.68 REMARK 500 3 GLU A 127 140.82 -34.90 REMARK 500 3 SER A 129 99.21 -38.99 REMARK 500 4 ARG A 32 -72.30 -48.16 REMARK 500 4 GLU A 57 101.12 -53.59 REMARK 500 4 PRO A 91 2.97 -69.75 REMARK 500 4 MET A 98 104.30 -49.31 REMARK 500 4 HIS A 99 40.91 -82.35 REMARK 500 4 HIS A 125 56.02 -110.59 REMARK 500 5 ASP A 8 -50.74 -127.88 REMARK 500 5 ASP A 9 74.17 -114.02 REMARK 500 5 ARG A 32 -72.91 -48.60 REMARK 500 5 ASP A 54 106.59 -59.00 REMARK 500 5 GLU A 57 95.56 -44.46 REMARK 500 5 PRO A 91 2.77 -69.77 REMARK 500 5 LEU A 93 -31.86 -36.97 REMARK 500 5 HIS A 99 46.37 -95.83 REMARK 500 6 GLU A 57 100.72 -53.74 REMARK 500 6 PRO A 91 2.40 -69.76 REMARK 500 6 HIS A 99 40.95 -95.82 REMARK 500 6 ASN A 124 -175.89 -54.34 REMARK 500 6 GLU A 127 71.57 -69.86 REMARK 500 6 SER A 129 -179.31 -173.24 REMARK 500 6 PRO A 131 87.09 -69.78 REMARK 500 7 SER A 3 92.75 -67.98 REMARK 500 7 GLU A 16 -37.96 -36.09 REMARK 500 7 GLU A 57 109.39 -38.49 REMARK 500 7 MET A 83 120.08 -35.03 REMARK 500 7 ASN A 85 179.38 -52.49 REMARK 500 7 PRO A 91 2.99 -69.75 REMARK 500 7 LEU A 93 -30.80 -36.42 REMARK 500 7 HIS A 125 40.73 -109.28 REMARK 500 REMARK 500 THIS ENTRY HAS 154 RAMACHANDRAN OUTLIERS. REMARK 500 REMARK 500 REMARK: NULL REMARK 900 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: HSK003000463.1 RELATED DB: TARGETDB DBREF 2D93 A 8 128 UNP Q8TEU7 RPGF6_HUMAN 265 385 SEQADV 2D93 GLY A 1 UNP Q8TEU7 CLONING ARTIFACT SEQADV 2D93 SER A 2 UNP Q8TEU7 CLONING ARTIFACT SEQADV 2D93 SER A 3 UNP Q8TEU7 CLONING ARTIFACT SEQADV 2D93 GLY A 4 UNP Q8TEU7 CLONING ARTIFACT SEQADV 2D93 SER A 5 UNP Q8TEU7 CLONING ARTIFACT SEQADV 2D93 SER A 6 UNP Q8TEU7 CLONING ARTIFACT SEQADV 2D93 GLY A 7 UNP Q8TEU7 CLONING ARTIFACT SEQADV 2D93 SER A 129 UNP Q8TEU7 CLONING ARTIFACT SEQADV 2D93 GLY A 130 UNP Q8TEU7 CLONING ARTIFACT SEQADV 2D93 PRO A 131 UNP Q8TEU7 CLONING ARTIFACT SEQADV 2D93 SER A 132 UNP Q8TEU7 CLONING ARTIFACT SEQADV 2D93 SER A 133 UNP Q8TEU7 CLONING ARTIFACT SEQADV 2D93 GLY A 134 UNP Q8TEU7 CLONING ARTIFACT SEQRES 1 A 134 GLY SER SER GLY SER SER GLY ASP ASP ASP ILE GLU GLN SEQRES 2 A 134 LEU LEU GLU PHE MET HIS GLN LEU PRO ALA PHE ALA ASN SEQRES 3 A 134 MET THR MET SER VAL ARG ARG GLU LEU CYS SER VAL MET SEQRES 4 A 134 ILE PHE GLU VAL VAL GLU GLN ALA GLY ALA ILE ILE LEU SEQRES 5 A 134 GLU ASP GLY GLN GLU LEU ASP SER TRP TYR VAL ILE LEU SEQRES 6 A 134 ASN GLY THR VAL GLU ILE SER HIS PRO ASP GLY LYS VAL SEQRES 7 A 134 GLU ASN LEU PHE MET GLY ASN SER PHE GLY ILE THR PRO SEQRES 8 A 134 THR LEU ASP LYS GLN TYR MET HIS GLY ILE VAL ARG THR SEQRES 9 A 134 LYS VAL ASP ASP CYS GLN PHE VAL CYS ILE ALA GLN GLN SEQRES 10 A 134 ASP TYR TRP ARG ILE LEU ASN HIS VAL GLU LYS SER GLY SEQRES 11 A 134 PRO SER SER GLY HELIX 1 1 ASP A 9 LEU A 21 1 13 HELIX 2 2 THR A 28 CYS A 36 1 9 HELIX 3 3 GLN A 116 LEU A 123 1 8 SHEET 1 A 4 MET A 39 VAL A 44 0 SHEET 2 A 4 CYS A 109 ALA A 115 -1 O CYS A 113 N ILE A 40 SHEET 3 A 4 SER A 60 VAL A 63 -1 N TRP A 61 O ILE A 114 SHEET 4 A 4 SER A 86 PHE A 87 -1 O PHE A 87 N TYR A 62 SHEET 1 B 4 ILE A 50 LEU A 52 0 SHEET 2 B 4 ILE A 101 THR A 104 -1 O VAL A 102 N LEU A 52 SHEET 3 B 4 VAL A 69 SER A 72 -1 N GLU A 70 O ARG A 103 SHEET 4 B 4 VAL A 78 LEU A 81 -1 O GLU A 79 N ILE A 71 SHEET 1 C 2 GLU A 57 LEU A 58 0 SHEET 2 C 2 GLN A 96 TYR A 97 -1 O GLN A 96 N LEU A 58 CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000 MODEL 1
Complete list - r 9 2 Bytes