Header list of 2b95.pdb file
Complete list - r 9 2 Bytes
HEADER TRANSPORT PROTEIN 10-OCT-05 2B95
TITLE SOLUTION NMR STRUCTURE OF PROTEIN DYNEIN LIGHT CHAIN 2A, CYTOPLASMIC;
TITLE 2 NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET HR2106
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: DYNEIN LIGHT CHAIN 2A;
COMPND 3 CHAIN: A, B;
COMPND 4 SYNONYM: DYNEIN-ASSOCIATED PROTEIN KM23, BITHORAXOID-LIKE PROTEIN,
COMPND 5 BLP;
COMPND 6 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE 3 ORGANISM_COMMON: HUMAN;
SOURCE 4 ORGANISM_TAXID: 9606;
SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE 6 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE 7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3)PMGK;
SOURCE 8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 9 EXPRESSION_SYSTEM_PLASMID: PHR2106.14.1 DERIVED FROM PET14
KEYWDS DYNEIN LIGHT CHAIN 2A, CYTOPLASMIC, HOMODIMER, STRUCTURAL GENOMICS,
KEYWDS 2 PSI, PROTEIN STRUCTURE INITIATIVE, NORTHEAST STRUCTURAL GENOMICS
KEYWDS 3 CONSORTIUM, NESG, TRANSPORT PROTEIN
EXPDTA SOLUTION NMR
NUMMDL 20
AUTHOR G.LIU,H.S.ATREYA,R.XIAO,T.ACTON,G.T.MONTELIONE,T.SZYPERSKI,NORTHEAST
AUTHOR 2 STRUCTURAL GENOMICS CONSORTIUM (NESG)
REVDAT 3 09-MAR-22 2B95 1 REMARK SEQADV
REVDAT 2 24-FEB-09 2B95 1 VERSN
REVDAT 1 01-NOV-05 2B95 0
SPRSDE 01-NOV-05 2B95 1TGQ
JRNL AUTH G.LIU,H.S.ATREYA,R.XIAO,T.ACTON,G.T.MONTELIONE,T.SZYPERSKI
JRNL TITL SOLUTION NMR STRUCTURE OF PROTEIN DYNEIN LIGHT CHAIN 2A,
JRNL TITL 2 CYTOPLASMIC; NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET
JRNL TITL 3 HR2106
JRNL REF TO BE PUBLISHED
JRNL REFN
REMARK 2
REMARK 2 RESOLUTION. NOT APPLICABLE.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : NMRPIPE 2.1, CYANA 2.1
REMARK 3 AUTHORS : DELAGLIO, F. (NMRPIPE), GUNTERT, P. (CYANA)
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 2B95 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 12-OCT-05.
REMARK 100 THE DEPOSITION ID IS D_1000034830.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210 EXPERIMENT TYPE : NMR
REMARK 210 TEMPERATURE (KELVIN) : 298
REMARK 210 PH : 6.0
REMARK 210 IONIC STRENGTH : NULL
REMARK 210 PRESSURE : AMBIENT
REMARK 210 SAMPLE CONTENTS : 1.3MM DYNEIN LIGHT CHAIN 2A, U
REMARK 210 -15N, 13C, 50 MM MES, 95% H2O, 5%
REMARK 210 D2O, 0.02% NAN3, PH 6.0
REMARK 210
REMARK 210 NMR EXPERIMENTS CONDUCTED : 3D_13C-SEPARATED_NOESY; 3D_15N
REMARK 210 -SEPARATED_NOESY; 3D_15N_13C_
REMARK 210 FILTER-EDIT SIMULTANEOUS NOESY;
REMARK 210 RD 3D HABCABCONHN; GFT (4,3)
REMARK 210 HNCABCA; GFT (4,3) CABCACONHN
REMARK 210 SPECTROMETER FIELD STRENGTH : 750 MHZ; 600 MHZ
REMARK 210 SPECTROMETER MODEL : INOVA
REMARK 210 SPECTROMETER MANUFACTURER : VARIAN
REMARK 210
REMARK 210 STRUCTURE DETERMINATION.
REMARK 210 SOFTWARE USED : XEASY 1.3.1, DYANA 1.5
REMARK 210 METHOD USED : DISTANCE GEOMETRY TORSION ANGLE
REMARK 210 DYNAMICS SIMULATED ANNEALING
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED : 100
REMARK 210 CONFORMERS, NUMBER SUBMITTED : 20
REMARK 210 CONFORMERS, SELECTION CRITERIA : STRUCTURES WITH THE LEAST
REMARK 210 RESTRAINT VIOLATIONS
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1
REMARK 210
REMARK 210 REMARK: THIS STRUCTURE WAS DETERMINED USING GFT/RD NMR TECHNIQUES
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 465 SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465 MODELS 1-20
REMARK 465 RES C SSSEQI
REMARK 465 MET A 1
REMARK 465 GLY A 2
REMARK 465 HIS A 3
REMARK 465 HIS A 4
REMARK 465 HIS A 5
REMARK 465 HIS A 6
REMARK 465 HIS A 7
REMARK 465 HIS A 8
REMARK 465 SER A 9
REMARK 465 HIS A 10
REMARK 465 MET B 1
REMARK 465 GLY B 2
REMARK 465 HIS B 3
REMARK 465 HIS B 4
REMARK 465 HIS B 5
REMARK 465 HIS B 6
REMARK 465 HIS B 7
REMARK 465 HIS B 8
REMARK 465 SER B 9
REMARK 465 HIS B 10
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 1 PRO A 39 89.12 -69.74
REMARK 500 1 MET A 44 -174.63 -64.55
REMARK 500 1 THR A 77 20.38 -153.08
REMARK 500 1 PHE A 78 132.04 -179.08
REMARK 500 1 ASP A 93 -168.59 -109.71
REMARK 500 1 ASP A 95 18.89 -153.47
REMARK 500 1 PRO B 39 82.15 -69.81
REMARK 500 1 MET B 44 -171.52 -68.81
REMARK 500 1 ASP B 75 116.96 -171.46
REMARK 500 1 THR B 77 17.18 -143.22
REMARK 500 1 PHE B 78 125.26 -171.42
REMARK 500 1 ASP B 93 -168.29 -111.96
REMARK 500 1 ASP B 95 19.15 -153.68
REMARK 500 2 PRO A 39 77.21 -69.76
REMARK 500 2 MET A 44 -171.34 -63.73
REMARK 500 2 LYS A 85 -66.29 -99.24
REMARK 500 2 ASP A 95 17.80 -151.22
REMARK 500 2 ASN B 34 -169.72 -67.55
REMARK 500 2 MET B 44 -168.76 -76.54
REMARK 500 2 ASP B 75 116.97 -167.29
REMARK 500 2 THR B 77 18.69 -142.49
REMARK 500 2 PHE B 78 123.53 -174.62
REMARK 500 2 ASN B 86 -178.94 -179.18
REMARK 500 2 ASP B 93 -168.52 -73.75
REMARK 500 2 ASP B 95 16.00 -148.47
REMARK 500 3 PRO A 39 82.19 -69.76
REMARK 500 3 MET A 44 -169.16 -68.07
REMARK 500 3 PHE A 78 121.86 -175.73
REMARK 500 3 ASP A 93 -168.70 -106.93
REMARK 500 3 ASP A 95 17.82 -151.51
REMARK 500 3 MET B 44 -171.73 -62.37
REMARK 500 3 PHE B 78 134.24 -179.31
REMARK 500 3 ASP B 93 -167.06 -103.58
REMARK 500 3 ASP B 95 18.89 -153.06
REMARK 500 4 PRO A 39 78.35 -69.77
REMARK 500 4 MET A 44 -168.68 -103.65
REMARK 500 4 ASP A 75 116.83 -162.00
REMARK 500 4 THR A 77 18.35 -143.73
REMARK 500 4 PHE A 78 124.07 -171.25
REMARK 500 4 ASP A 95 16.40 -149.00
REMARK 500 4 ASN B 34 -169.82 -102.70
REMARK 500 4 PRO B 39 83.49 -69.80
REMARK 500 4 MET B 44 -168.30 -100.45
REMARK 500 4 THR B 77 15.49 -141.78
REMARK 500 4 PHE B 78 141.58 -179.46
REMARK 500 4 ASP B 95 19.23 -153.76
REMARK 500 5 ASN A 34 -169.91 -67.20
REMARK 500 5 SER A 42 111.42 -165.17
REMARK 500 5 MET A 44 -169.54 -61.30
REMARK 500 5 SER A 83 -169.97 -106.58
REMARK 500
REMARK 500 THIS ENTRY HAS 238 RAMACHANDRAN OUTLIERS.
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: HR2106 RELATED DB: TARGETDB
DBREF 2B95 A 11 106 UNP Q9NP97 DLC2A_HUMAN 0 95
DBREF 2B95 B 11 106 UNP Q9NP97 DLC2A_HUMAN 0 95
SEQADV 2B95 MET A 1 UNP Q9NP97 CLONING ARTIFACT
SEQADV 2B95 GLY A 2 UNP Q9NP97 CLONING ARTIFACT
SEQADV 2B95 HIS A 3 UNP Q9NP97 EXPRESSION TAG
SEQADV 2B95 HIS A 4 UNP Q9NP97 EXPRESSION TAG
SEQADV 2B95 HIS A 5 UNP Q9NP97 EXPRESSION TAG
SEQADV 2B95 HIS A 6 UNP Q9NP97 EXPRESSION TAG
SEQADV 2B95 HIS A 7 UNP Q9NP97 EXPRESSION TAG
SEQADV 2B95 HIS A 8 UNP Q9NP97 EXPRESSION TAG
SEQADV 2B95 SER A 9 UNP Q9NP97 CLONING ARTIFACT
SEQADV 2B95 HIS A 10 UNP Q9NP97 CLONING ARTIFACT
SEQADV 2B95 MET B 1 UNP Q9NP97 CLONING ARTIFACT
SEQADV 2B95 GLY B 2 UNP Q9NP97 CLONING ARTIFACT
SEQADV 2B95 HIS B 3 UNP Q9NP97 EXPRESSION TAG
SEQADV 2B95 HIS B 4 UNP Q9NP97 EXPRESSION TAG
SEQADV 2B95 HIS B 5 UNP Q9NP97 EXPRESSION TAG
SEQADV 2B95 HIS B 6 UNP Q9NP97 EXPRESSION TAG
SEQADV 2B95 HIS B 7 UNP Q9NP97 EXPRESSION TAG
SEQADV 2B95 HIS B 8 UNP Q9NP97 EXPRESSION TAG
SEQADV 2B95 SER B 9 UNP Q9NP97 CLONING ARTIFACT
SEQADV 2B95 HIS B 10 UNP Q9NP97 CLONING ARTIFACT
SEQRES 1 A 106 MET GLY HIS HIS HIS HIS HIS HIS SER HIS MET ALA GLU
SEQRES 2 A 106 VAL GLU GLU THR LEU LYS ARG LEU GLN SER GLN LYS GLY
SEQRES 3 A 106 VAL GLN GLY ILE ILE VAL VAL ASN THR GLU GLY ILE PRO
SEQRES 4 A 106 ILE LYS SER THR MET ASP ASN PRO THR THR THR GLN TYR
SEQRES 5 A 106 ALA SER LEU MET HIS SER PHE ILE LEU LYS ALA ARG SER
SEQRES 6 A 106 THR VAL ARG ASP ILE ASP PRO GLN ASN ASP LEU THR PHE
SEQRES 7 A 106 LEU ARG ILE ARG SER LYS LYS ASN GLU ILE MET VAL ALA
SEQRES 8 A 106 PRO ASP LYS ASP TYR PHE LEU ILE VAL ILE GLN ASN PRO
SEQRES 9 A 106 THR GLU
SEQRES 1 B 106 MET GLY HIS HIS HIS HIS HIS HIS SER HIS MET ALA GLU
SEQRES 2 B 106 VAL GLU GLU THR LEU LYS ARG LEU GLN SER GLN LYS GLY
SEQRES 3 B 106 VAL GLN GLY ILE ILE VAL VAL ASN THR GLU GLY ILE PRO
SEQRES 4 B 106 ILE LYS SER THR MET ASP ASN PRO THR THR THR GLN TYR
SEQRES 5 B 106 ALA SER LEU MET HIS SER PHE ILE LEU LYS ALA ARG SER
SEQRES 6 B 106 THR VAL ARG ASP ILE ASP PRO GLN ASN ASP LEU THR PHE
SEQRES 7 B 106 LEU ARG ILE ARG SER LYS LYS ASN GLU ILE MET VAL ALA
SEQRES 8 B 106 PRO ASP LYS ASP TYR PHE LEU ILE VAL ILE GLN ASN PRO
SEQRES 9 B 106 THR GLU
HELIX 1 1 MET A 11 GLN A 24 1 14
HELIX 2 2 ASP A 45 ASP A 71 1 27
HELIX 3 3 MET B 11 GLN B 24 1 14
HELIX 4 4 ASP B 45 ASP B 71 1 27
SHEET 1 A10 PRO A 39 SER A 42 0
SHEET 2 A10 VAL A 27 ASN A 34 -1 N VAL A 32 O LYS A 41
SHEET 3 A10 TYR A 96 GLN A 102 -1 O PHE A 97 N VAL A 33
SHEET 4 A10 GLU A 87 PRO A 92 -1 N MET A 89 O VAL A 100
SHEET 5 A10 LEU A 76 ARG A 82 -1 N LEU A 79 O VAL A 90
SHEET 6 A10 PHE B 78 SER B 83 -1 O ARG B 82 N THR A 77
SHEET 7 A10 GLU B 87 PRO B 92 -1 O VAL B 90 N LEU B 79
SHEET 8 A10 TYR B 96 GLN B 102 -1 O VAL B 100 N MET B 89
SHEET 9 A10 VAL B 27 ASN B 34 -1 N VAL B 33 O PHE B 97
SHEET 10 A10 PRO B 39 SER B 42 -1 O LYS B 41 N VAL B 32
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
MODEL 1
Complete list - r 9 2 Bytes