Header list of 2aqa.pdb file
Complete list - r 9 2 Bytes
HEADER RNA BINDING PROTEIN 17-AUG-05 2AQA TITLE NMR STRUCTURAL ANALYSIS OF NOP10P FROM SACCHAROMYCES CEREVISIAE COMPND MOL_ID: 1; COMPND 2 MOLECULE: H/ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 3; COMPND 3 CHAIN: A; COMPND 4 SYNONYM: NUCLEOLAR PROTEIN FAMILY A MEMBER 3, SNORNP PROTEIN NOP10; COMPND 5 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE; SOURCE 3 ORGANISM_COMMON: BAKER'S YEAST; SOURCE 4 ORGANISM_TAXID: 4932; SOURCE 5 GENE: NOP10; SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 7 EXPRESSION_SYSTEM_TAXID: 562; SOURCE 8 EXPRESSION_SYSTEM_STRAIN: ROSETTA PLYS S; SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 10 EXPRESSION_SYSTEM_PLASMID: PET9 KEYWDS NOP10P, RNA BINDING PROTEIN EXPDTA SOLUTION NMR NUMMDL 20 AUTHOR T.HAMMA,S.L.REICHOW,G.VARANI,A.R.FERRE-D'AMARE REVDAT 4 09-MAR-22 2AQA 1 REMARK SEQADV REVDAT 3 24-FEB-09 2AQA 1 VERSN REVDAT 2 03-JAN-06 2AQA 1 JRNL REVDAT 1 15-NOV-05 2AQA 0 JRNL AUTH T.HAMMA,S.L.REICHOW,G.VARANI,A.R.FERRE-D'AMARE JRNL TITL THE CBF5-NOP10 COMPLEX IS A MOLECULAR BRACKET THAT ORGANIZES JRNL TITL 2 BOX H/ACA RNPS. JRNL REF NAT.STRUCT.MOL.BIOL. V. 12 1101 2005 JRNL REFN ISSN 1545-9993 JRNL PMID 16286935 JRNL DOI 10.1038/NSMB1036 REMARK 2 REMARK 2 RESOLUTION. NOT APPLICABLE. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 PROGRAM : CYANA V. 2.0 REMARK 3 AUTHORS : GUNTERT, P. REMARK 3 REMARK 3 OTHER REFINEMENT REMARKS: REMARK 3 THE STRUCTURES ARE BASED ON A TOTAL OF 467 RESTRAINTS, 422 ARE NOE- REMARK 3 DERIVED REMARK 3 DISTANCE CONSTRAINTS, 45 DIHEDRAL ANGLE RESTRAINTS REMARK 4 REMARK 4 2AQA COMPLIES WITH FORMAT V. 3.15, 01-DEC-08 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 18-AUG-05. REMARK 100 THE DEPOSITION ID IS D_1000034186. REMARK 210 REMARK 210 EXPERIMENTAL DETAILS REMARK 210 EXPERIMENT TYPE : NMR REMARK 210 TEMPERATURE (KELVIN) : 298 REMARK 210 PH : 5.0 REMARK 210 IONIC STRENGTH : 50MM; 100MM REMARK 210 PRESSURE : 1 ATM REMARK 210 SAMPLE CONTENTS : 1MM YNOP10P UNLABELLED; SODIUM REMARK 210 ACETATE, KCL; 1MM YNOP10P REMARK 210 UNLABELLED; SODIUM ACETATE, KCL; REMARK 210 1MM YNOP10P 15N-LABELLED; SODIUM REMARK 210 ACETATE, KCL; 1MM YNOP10P 15N, REMARK 210 13C-LABELLED; SODIUM ACETATE, KCL REMARK 210 REMARK 210 NMR EXPERIMENTS CONDUCTED : 2D NOESY; 3D_15N REMARK 210 -SEPARATED_NOESY; 3D_13C- REMARK 210 SEPARATED_NOESY REMARK 210 SPECTROMETER FIELD STRENGTH : 500 MHZ; 750 MHZ; 800 MHZ REMARK 210 SPECTROMETER MODEL : DRX; DMX; INOVA REMARK 210 SPECTROMETER MANUFACTURER : BRUKER; VARIAN REMARK 210 REMARK 210 STRUCTURE DETERMINATION. REMARK 210 SOFTWARE USED : NMRPIPE V. 2.3, SPARKY V. 3.110, REMARK 210 TALOS V. 2003.027.13.05 REMARK 210 METHOD USED : TORSION ANGLE DYNAMICS REMARK 210 REMARK 210 CONFORMERS, NUMBER CALCULATED : 100 REMARK 210 CONFORMERS, NUMBER SUBMITTED : 20 REMARK 210 CONFORMERS, SELECTION CRITERIA : STRUCTURES WITH THE LEAST REMARK 210 RESTRAINT VIOLATIONS REMARK 210 REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1 REMARK 210 REMARK 210 REMARK: DIHEDRAL RESTRAINTS DERIVED FROM TALOS PREDICITONS REMARK 215 REMARK 215 NMR STUDY REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON REMARK 215 THESE RECORDS ARE MEANINGLESS. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC REMARK 350 APPLY THE FOLLOWING TO CHAINS: A REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 465 REMARK 465 MISSING RESIDUES REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE REMARK 465 EXPERIMENT. (RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 465 SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.) REMARK 465 MODELS 1-20 REMARK 465 RES C SSSEQI REMARK 465 HIS A 59 REMARK 465 HIS A 60 REMARK 465 HIS A 61 REMARK 465 HIS A 62 REMARK 465 HIS A 63 REMARK 465 HIS A 64 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2) REMARK 500 REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI- REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400 REMARK 500 REMARK 500 M RES CSSEQI PSI PHI REMARK 500 1 TYR A 15 95.93 -61.33 REMARK 500 1 THR A 21 -73.68 -55.77 REMARK 500 1 SER A 36 68.53 -168.52 REMARK 500 1 LEU A 48 175.04 -54.91 REMARK 500 2 TYR A 15 84.52 -69.85 REMARK 500 2 THR A 16 32.53 -146.41 REMARK 500 2 HIS A 31 73.29 58.00 REMARK 500 2 SER A 36 70.70 -177.67 REMARK 500 2 LEU A 48 -74.71 -56.04 REMARK 500 3 LYS A 19 174.02 -59.59 REMARK 500 3 SER A 29 34.22 -96.38 REMARK 500 3 HIS A 31 72.50 58.75 REMARK 500 3 PRO A 32 -173.45 -69.77 REMARK 500 3 VAL A 55 71.70 -115.67 REMARK 500 4 TYR A 15 109.24 -53.04 REMARK 500 4 THR A 16 28.54 -152.42 REMARK 500 4 HIS A 31 71.45 -171.68 REMARK 500 4 ARG A 34 131.24 -176.55 REMARK 500 4 SER A 36 72.42 -173.22 REMARK 500 4 PHE A 52 104.86 -56.49 REMARK 500 5 TYR A 15 105.64 -58.37 REMARK 500 5 THR A 16 25.63 -149.96 REMARK 500 5 HIS A 31 153.78 64.51 REMARK 500 5 PRO A 32 -175.50 -69.79 REMARK 500 5 ARG A 43 -72.83 -52.09 REMARK 500 6 TYR A 15 100.51 -58.36 REMARK 500 6 SER A 23 77.29 -115.48 REMARK 500 6 HIS A 31 68.47 -163.40 REMARK 500 6 LYS A 49 -74.64 -83.75 REMARK 500 7 TYR A 15 99.55 -57.53 REMARK 500 7 PRO A 32 -172.01 -69.83 REMARK 500 7 SER A 36 73.05 -165.87 REMARK 500 7 ASP A 38 102.00 -59.25 REMARK 500 7 LYS A 40 -77.73 -95.30 REMARK 500 7 VAL A 55 69.44 61.43 REMARK 500 8 TYR A 15 107.13 -51.61 REMARK 500 8 LYS A 18 105.02 -53.62 REMARK 500 8 ARG A 34 147.00 -175.49 REMARK 500 8 VAL A 55 71.22 62.20 REMARK 500 9 TYR A 15 103.54 -51.43 REMARK 500 9 PRO A 32 99.98 -69.79 REMARK 500 10 MET A 4 33.49 -95.72 REMARK 500 10 LYS A 18 103.67 -58.57 REMARK 500 10 LYS A 50 109.13 -51.66 REMARK 500 11 TYR A 15 104.48 -51.79 REMARK 500 11 LEU A 48 117.07 -166.82 REMARK 500 11 LYS A 50 -179.47 -57.45 REMARK 500 11 VAL A 55 72.73 58.67 REMARK 500 12 TYR A 15 99.16 -57.46 REMARK 500 12 THR A 16 24.49 -144.43 REMARK 500 REMARK 500 THIS ENTRY HAS 86 RAMACHANDRAN OUTLIERS. REMARK 500 REMARK 500 REMARK: NULL DBREF 2AQA A 2 58 UNP Q6Q547 NOP10_YEAST 2 58 SEQADV 2AQA HIS A 59 UNP Q6Q547 EXPRESSION TAG SEQADV 2AQA HIS A 60 UNP Q6Q547 EXPRESSION TAG SEQADV 2AQA HIS A 61 UNP Q6Q547 EXPRESSION TAG SEQADV 2AQA HIS A 62 UNP Q6Q547 EXPRESSION TAG SEQADV 2AQA HIS A 63 UNP Q6Q547 EXPRESSION TAG SEQADV 2AQA HIS A 64 UNP Q6Q547 EXPRESSION TAG SEQRES 1 A 63 HIS LEU MET TYR THR LEU GLY PRO ASP GLY LYS ARG ILE SEQRES 2 A 63 TYR THR LEU LYS LYS VAL THR GLU SER GLY GLU ILE THR SEQRES 3 A 63 LYS SER ALA HIS PRO ALA ARG PHE SER PRO ASP ASP LYS SEQRES 4 A 63 TYR SER ARG GLN ARG VAL THR LEU LYS LYS ARG PHE GLY SEQRES 5 A 63 LEU VAL PRO GLY GLN HIS HIS HIS HIS HIS HIS HELIX 1 1 SER A 42 THR A 47 1 6 SHEET 1 A 2 THR A 6 LEU A 7 0 SHEET 2 A 2 ARG A 13 ILE A 14 -1 O ILE A 14 N THR A 6 CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000 MODEL 1
Complete list - r 9 2 Bytes