Header list of 1y2y.pdb file
Complete list - 2 20 Bytes
HEADER BIOSYNTHETIC PROTEIN 23-NOV-04 1Y2Y TITLE STRUCTURAL CHARACTERIZATION OF NOP10P USING NUCLEAR MAGNETIC RESONANCE TITLE 2 SPECTROSCOPY COMPND MOL_ID: 1; COMPND 2 MOLECULE: RIBOSOME BIOGENESIS PROTEIN NOP10; COMPND 3 CHAIN: A; COMPND 4 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE; SOURCE 3 ORGANISM_COMMON: BAKER'S YEAST; SOURCE 4 ORGANISM_TAXID: 4932; SOURCE 5 GENE: NOP10; SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 7 EXPRESSION_SYSTEM_TAXID: 562; SOURCE 8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3) GOLD; SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PET15B KEYWDS BOX H/ACA SNORNA, SNORNP, PSEUDOURIDINE, BIOSYNTHETIC PROTEIN EXPDTA SOLUTION NMR NUMMDL 10 AUTHOR M.KHANNA,H.WU,C.JOHANSSON,M.CAIZERGUES-FERRER,J.FEIGON REVDAT 4 02-MAR-22 1Y2Y 1 REMARK REVDAT 3 24-FEB-09 1Y2Y 1 VERSN REVDAT 2 10-JAN-06 1Y2Y 1 JRNL REVDAT 1 06-DEC-05 1Y2Y 0 JRNL AUTH M.KHANNA,H.WU,C.JOHANSSON,M.CAIZERGUES-FERRER,J.FEIGON JRNL TITL STRUCTURAL STUDY OF THE H/ACA SNORNP COMPONENTS NOP10P AND JRNL TITL 2 THE 3' HAIRPIN OF U65 SNORNA JRNL REF RNA V. 12 40 2006 JRNL REFN ISSN 1355-8382 JRNL PMID 16373493 JRNL DOI 10.1261/RNA.2221606 REMARK 2 REMARK 2 RESOLUTION. NOT APPLICABLE. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 PROGRAM : XWINNMR 3.5, X-PLOR 3.1 REMARK 3 AUTHORS : BRUKER (XWINNMR), NIH (X-PLOR) REMARK 3 REMARK 3 OTHER REFINEMENT REMARKS: THE STRUCTURES ARE BASED ON 354 REMARK 3 INTRARESIDUE NOES, 191 SEQUENTIAL NOES AND 10 HYDROGEN BOND REMARK 3 RESTRAINTS. REMARK 4 REMARK 4 1Y2Y COMPLIES WITH FORMAT V. 3.15, 01-DEC-08 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 24-NOV-04. REMARK 100 THE DEPOSITION ID IS D_1000031049. REMARK 210 REMARK 210 EXPERIMENTAL DETAILS REMARK 210 EXPERIMENT TYPE : NMR REMARK 210 TEMPERATURE (KELVIN) : 288 REMARK 210 PH : 6.5 REMARK 210 IONIC STRENGTH : 110 MM REMARK 210 PRESSURE : AMBIENT REMARK 210 SAMPLE CONTENTS : 1.5 MM 15N/13C NOP1P0P; 10 MM REMARK 210 POTASSIUM PHOSPHATE BUFFER, PH REMARK 210 6.5; 100 MM POTASSIUM CHLORIDE; REMARK 210 90% H2O, 10% D2O; 1.5 MM 15N/13C REMARK 210 NOP1P0P; 10 MM POTASSIUM REMARK 210 PHOSPHATE BUFFER, PH 6.5; 100 MM REMARK 210 POTASSIUM CHLORIDE; 100% D2O REMARK 210 REMARK 210 NMR EXPERIMENTS CONDUCTED : 3D_15N-SEPARATED_NOESY; 3D_13C REMARK 210 -SEPARATED_NOESY; 2D TOCSY; 3D REMARK 210 HCCH-TOCSY; CBCANH; CBCA(CO)NH REMARK 210 SPECTROMETER FIELD STRENGTH : 500 MHZ; 600 MHZ REMARK 210 SPECTROMETER MODEL : DRX REMARK 210 SPECTROMETER MANUFACTURER : BRUKER REMARK 210 REMARK 210 STRUCTURE DETERMINATION. REMARK 210 SOFTWARE USED : XWINNMR 3.5, AURELIA 3.0, X-PLOR REMARK 210 3.1 REMARK 210 METHOD USED : REFINEMENT WITH SIMULATED REMARK 210 ANNEALING REMARK 210 REMARK 210 CONFORMERS, NUMBER CALCULATED : 30 REMARK 210 CONFORMERS, NUMBER SUBMITTED : 10 REMARK 210 CONFORMERS, SELECTION CRITERIA : STRUCTURES WITH THE LOWEST REMARK 210 ENERGY REMARK 210 REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 10 REMARK 210 REMARK 210 REMARK: NULL REMARK 215 REMARK 215 NMR STUDY REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON REMARK 215 THESE RECORDS ARE MEANINGLESS. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC REMARK 350 APPLY THE FOLLOWING TO CHAINS: A REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: CLOSE CONTACTS REMARK 500 REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT. REMARK 500 REMARK 500 ATM1 RES C SSEQI ATM2 RES C SSEQI DISTANCE REMARK 500 O VAL A 55 H GLY A 57 1.45 REMARK 500 OG1 THR A 47 H LEU A 48 1.59 REMARK 500 REMARK 500 REMARK: NULL REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2) REMARK 500 REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI- REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400 REMARK 500 REMARK 500 M RES CSSEQI PSI PHI REMARK 500 1 LEU A 3 117.18 67.87 REMARK 500 1 ASP A 10 -78.59 -117.44 REMARK 500 1 LYS A 12 -75.26 -141.34 REMARK 500 1 ARG A 13 47.32 175.05 REMARK 500 1 ILE A 14 101.24 -36.56 REMARK 500 1 THR A 16 -162.78 -164.79 REMARK 500 1 LYS A 19 171.79 -49.69 REMARK 500 1 SER A 23 -179.57 50.33 REMARK 500 1 LYS A 28 118.95 153.39 REMARK 500 1 SER A 29 -148.62 31.10 REMARK 500 1 ALA A 30 176.76 48.64 REMARK 500 1 ALA A 33 -24.82 161.12 REMARK 500 1 PHE A 35 99.23 -35.71 REMARK 500 1 SER A 36 -71.27 -141.52 REMARK 500 1 ASP A 39 7.74 80.50 REMARK 500 1 LYS A 40 9.63 84.14 REMARK 500 1 TYR A 41 31.69 -99.84 REMARK 500 1 ARG A 43 102.60 46.17 REMARK 500 1 ARG A 45 -6.54 85.55 REMARK 500 1 THR A 47 -94.28 62.68 REMARK 500 1 LEU A 48 22.75 -159.13 REMARK 500 1 ARG A 51 175.90 50.11 REMARK 500 1 LEU A 54 170.58 52.48 REMARK 500 1 VAL A 55 -59.23 -166.85 REMARK 500 1 PRO A 56 60.16 -56.00 REMARK 500 2 LEU A 3 80.82 52.89 REMARK 500 2 ASP A 10 23.57 170.37 REMARK 500 2 ILE A 14 92.00 -33.49 REMARK 500 2 THR A 16 100.05 -168.24 REMARK 500 2 LEU A 17 49.46 -82.86 REMARK 500 2 LYS A 18 -63.19 65.44 REMARK 500 2 LYS A 19 -84.90 22.87 REMARK 500 2 VAL A 20 -65.10 129.02 REMARK 500 2 THR A 21 88.25 -68.97 REMARK 500 2 GLU A 22 -63.52 -156.09 REMARK 500 2 LYS A 28 124.22 127.52 REMARK 500 2 SER A 29 -161.18 78.24 REMARK 500 2 HIS A 31 52.84 -153.65 REMARK 500 2 ALA A 33 -11.13 142.90 REMARK 500 2 ARG A 34 12.08 -141.82 REMARK 500 2 PHE A 35 134.99 53.14 REMARK 500 2 SER A 36 -40.85 157.84 REMARK 500 2 PRO A 37 -173.63 -44.82 REMARK 500 2 ASP A 38 -32.86 -37.56 REMARK 500 2 TYR A 41 -78.17 -57.04 REMARK 500 2 SER A 42 139.09 164.10 REMARK 500 2 ARG A 45 -57.32 -129.08 REMARK 500 2 VAL A 46 -45.77 -145.32 REMARK 500 2 LEU A 48 46.69 -109.86 REMARK 500 2 PHE A 52 -8.06 83.34 REMARK 500 REMARK 500 THIS ENTRY HAS 287 RAMACHANDRAN OUTLIERS. REMARK 500 REMARK 500 REMARK: NULL DBREF 1Y2Y A 1 58 UNP Q6Q547 NOP10_YEAST 1 58 SEQRES 1 A 58 MET HIS LEU MET TYR THR LEU GLY PRO ASP GLY LYS ARG SEQRES 2 A 58 ILE TYR THR LEU LYS LYS VAL THR GLU SER GLY GLU ILE SEQRES 3 A 58 THR LYS SER ALA HIS PRO ALA ARG PHE SER PRO ASP ASP SEQRES 4 A 58 LYS TYR SER ARG GLN ARG VAL THR LEU LYS LYS ARG PHE SEQRES 5 A 58 GLY LEU VAL PRO GLY GLN CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000 MODEL 1
Complete list - 2 20 Bytes