Header list of 1q1o.pdb file
Complete list - r 2 2 Bytes
HEADER SIGNALING PROTEIN 22-JUL-03 1Q1O TITLE SOLUTION STRUCTURE OF THE PB1 DOMAIN OF CDC24P (LONG FORM) COMPND MOL_ID: 1; COMPND 2 MOLECULE: CELL DIVISION CONTROL PROTEIN 24; COMPND 3 CHAIN: A; COMPND 4 FRAGMENT: PB1 DOMAIN (LONG FORM); COMPND 5 SYNONYM: CDC24P, CALCIUM REGULATORY PROTEIN; COMPND 6 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE; SOURCE 3 ORGANISM_COMMON: BAKER'S YEAST; SOURCE 4 ORGANISM_TAXID: 4932; SOURCE 5 GENE: CDC24P; SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3); SOURCE 7 EXPRESSION_SYSTEM_TAXID: 469008; SOURCE 8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3); SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 10 EXPRESSION_SYSTEM_PLASMID: PGEX6P-1 KEYWDS PB1 DOMAIN, PCCR, PC MOTIF, OPCA MOTIF, YEAST, CELL POLARITY, KEYWDS 2 PROTEIN-PROTEIN INTERACTION, SIGNALING PROTEIN EXPDTA SOLUTION NMR NUMMDL 20 AUTHOR S.YOSHINAGA,M.KOHJIMA,K.OGURA,M.YOKOCHI,R.TAKEYA,T.ITO,H.SUMIMOTO, AUTHOR 2 F.INAGAKI REVDAT 3 02-MAR-22 1Q1O 1 REMARK SEQADV REVDAT 2 24-FEB-09 1Q1O 1 VERSN REVDAT 1 14-OCT-03 1Q1O 0 JRNL AUTH S.YOSHINAGA,M.KOHJIMA,K.OGURA,M.YOKOCHI,R.TAKEYA,T.ITO, JRNL AUTH 2 H.SUMIMOTO,F.INAGAKI JRNL TITL THE PB1 DOMAIN AND THE PC MOTIF-CONTAINING REGION ARE JRNL TITL 2 STRUCTURALLY SIMILAR PROTEIN BINDING MODULES JRNL REF EMBO J. V. 22 4888 2003 JRNL REFN ISSN 0261-4189 JRNL PMID 14517229 JRNL DOI 10.1093/EMBOJ/CDG475 REMARK 2 REMARK 2 RESOLUTION. NOT APPLICABLE. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 PROGRAM : VNMR, ARIA REMARK 3 AUTHORS : J.P.LINGE, S.I.O'DONOGHUE, M.NILGES (ARIA) REMARK 3 REMARK 3 OTHER REFINEMENT REMARKS: NULL REMARK 4 REMARK 4 1Q1O COMPLIES WITH FORMAT V. 3.15, 01-DEC-08 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 24-JUL-03. REMARK 100 THE DEPOSITION ID IS D_1000019804. REMARK 210 REMARK 210 EXPERIMENTAL DETAILS REMARK 210 EXPERIMENT TYPE : NMR REMARK 210 TEMPERATURE (KELVIN) : 298 REMARK 210 PH : 6.3 REMARK 210 IONIC STRENGTH : 50MM POTASSIUM PHOSPHATE BUFFER; REMARK 210 150MM SODIUM CHLORIDE REMARK 210 PRESSURE : AMBIENT REMARK 210 SAMPLE CONTENTS : 1MM CDC24P PB1 U-15N ,13C; 50MM REMARK 210 POTASSIUM PHOSPHATE BUFFER; REMARK 210 150MM SODIUM CHLORIDE; 1MM REMARK 210 SODIUM AZIDE; 90% H2O, 10% D2O; REMARK 210 1MM CDC24P PB1 U-15N, 13C; 50MM REMARK 210 POTASSIUM PHOSPHATE BUFFER; REMARK 210 150MM SODIUM CHLORIDE; 1MM REMARK 210 SODIUM AZIDE; 100% D2O REMARK 210 REMARK 210 NMR EXPERIMENTS CONDUCTED : 3D_15N-SEPARATED_NOESY; 3D_13C REMARK 210 -SEPARATED_NOESY REMARK 210 SPECTROMETER FIELD STRENGTH : 600 MHZ; 500 MHZ REMARK 210 SPECTROMETER MODEL : UNITYPLUS; INOVA REMARK 210 SPECTROMETER MANUFACTURER : VARIAN REMARK 210 REMARK 210 STRUCTURE DETERMINATION. REMARK 210 SOFTWARE USED : NMRPIPE, OLIVIA, ARIA, CNS REMARK 210 METHOD USED : SIMULATED ANNEALING REMARK 210 REMARK 210 CONFORMERS, NUMBER CALCULATED : 100 REMARK 210 CONFORMERS, NUMBER SUBMITTED : 20 REMARK 210 CONFORMERS, SELECTION CRITERIA : STRUCTURES WITH THE LOWEST REMARK 210 ENERGY REMARK 210 REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1 REMARK 210 REMARK 210 REMARK: NULL REMARK 215 REMARK 215 NMR STUDY REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON REMARK 215 THESE RECORDS ARE MEANINGLESS. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC REMARK 350 APPLY THE FOLLOWING TO CHAINS: A REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2) REMARK 500 REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI- REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400 REMARK 500 REMARK 500 M RES CSSEQI PSI PHI REMARK 500 1 SER A 772 88.01 56.94 REMARK 500 1 SER A 776 -16.03 -161.15 REMARK 500 1 SER A 777 85.32 56.24 REMARK 500 1 THR A 814 11.79 -144.28 REMARK 500 1 GLU A 846 -171.72 -66.71 REMARK 500 1 LYS A 847 -27.95 -157.78 REMARK 500 2 SER A 761 -168.41 -121.40 REMARK 500 2 ASN A 773 78.73 58.47 REMARK 500 2 THR A 775 -70.88 -134.70 REMARK 500 2 SER A 776 54.39 -151.72 REMARK 500 2 SER A 803 59.63 -103.12 REMARK 500 2 ASN A 804 -41.93 -141.54 REMARK 500 2 HIS A 806 -168.65 -76.91 REMARK 500 2 ASN A 807 -72.03 -59.39 REMARK 500 2 ASN A 808 -74.74 -140.79 REMARK 500 2 PRO A 812 176.84 -57.84 REMARK 500 2 LYS A 815 93.19 -165.90 REMARK 500 2 ASP A 820 -164.72 -113.27 REMARK 500 2 LYS A 847 -28.04 -147.69 REMARK 500 3 SER A 761 174.77 60.99 REMARK 500 3 ASN A 771 -159.90 -109.52 REMARK 500 3 SER A 772 -55.00 -130.18 REMARK 500 3 SER A 776 105.75 -166.87 REMARK 500 3 SER A 777 18.99 -145.27 REMARK 500 3 ASN A 804 -35.02 -174.58 REMARK 500 3 THR A 805 -161.60 -101.70 REMARK 500 3 HIS A 806 -165.85 59.31 REMARK 500 3 ASN A 808 -85.00 -124.50 REMARK 500 3 ASN A 809 136.50 -171.28 REMARK 500 3 THR A 814 -56.00 -148.08 REMARK 500 3 ASN A 845 14.92 59.11 REMARK 500 4 ASN A 771 -78.12 -105.98 REMARK 500 4 SER A 772 -68.59 -158.53 REMARK 500 4 ASN A 773 -4.27 -168.49 REMARK 500 4 SER A 776 -167.91 57.16 REMARK 500 4 ASN A 804 -32.95 -164.25 REMARK 500 4 PRO A 812 -176.74 -66.67 REMARK 500 4 ASN A 845 16.56 58.24 REMARK 500 5 SER A 761 -177.38 59.64 REMARK 500 5 SER A 772 92.16 -162.96 REMARK 500 5 ASN A 774 -163.88 54.38 REMARK 500 5 SER A 776 88.23 -157.65 REMARK 500 5 SER A 777 159.50 63.67 REMARK 500 5 ASN A 808 7.38 -164.08 REMARK 500 6 PRO A 758 -90.35 -71.52 REMARK 500 6 LEU A 759 -30.31 -157.12 REMARK 500 6 SER A 772 -170.72 57.26 REMARK 500 6 ASN A 773 86.65 55.50 REMARK 500 6 ASN A 774 -170.11 59.53 REMARK 500 6 SER A 776 -56.65 -161.55 REMARK 500 REMARK 500 THIS ENTRY HAS 166 RAMACHANDRAN OUTLIERS. REMARK 500 REMARK 500 REMARK: NULL REMARK 900 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: 1IP9 RELATED DB: PDB REMARK 900 THE SAME DOMAIN FAMILY AND THE BINDING PARTNER REMARK 900 RELATED ID: 1IPG RELATED DB: PDB REMARK 900 THE SAME DOMAIN FAMILY AND THE BINDING PARTNER DBREF 1Q1O A 761 854 UNP P11433 CDC24_YEAST 761 854 SEQADV 1Q1O GLY A 757 UNP P11433 CLONING ARTIFACT SEQADV 1Q1O PRO A 758 UNP P11433 CLONING ARTIFACT SEQADV 1Q1O LEU A 759 UNP P11433 CLONING ARTIFACT SEQADV 1Q1O GLY A 760 UNP P11433 CLONING ARTIFACT SEQRES 1 A 98 GLY PRO LEU GLY SER ILE LEU PHE ARG ILE SER TYR ASN SEQRES 2 A 98 ASN ASN SER ASN ASN THR SER SER SER GLU ILE PHE THR SEQRES 3 A 98 LEU LEU VAL GLU LYS VAL TRP ASN PHE ASP ASP LEU ILE SEQRES 4 A 98 MET ALA ILE ASN SER LYS ILE SER ASN THR HIS ASN ASN SEQRES 5 A 98 ASN ILE SER PRO ILE THR LYS ILE LYS TYR GLN ASP GLU SEQRES 6 A 98 ASP GLY ASP PHE VAL VAL LEU GLY SER ASP GLU ASP TRP SEQRES 7 A 98 ASN VAL ALA LYS GLU MET LEU ALA GLU ASN ASN GLU LYS SEQRES 8 A 98 PHE LEU ASN ILE ARG LEU TYR HELIX 1 1 ASN A 790 HIS A 806 1 17 HELIX 2 2 SER A 830 ASN A 844 1 15 SHEET 1 A 5 GLU A 779 VAL A 785 0 SHEET 2 A 5 ILE A 762 TYR A 768 -1 N ILE A 762 O VAL A 785 SHEET 3 A 5 PHE A 848 LEU A 853 1 O LEU A 849 N ARG A 765 SHEET 4 A 5 ILE A 816 GLN A 819 -1 N LYS A 817 O ARG A 852 SHEET 5 A 5 PHE A 825 LEU A 828 -1 O VAL A 826 N TYR A 818 CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000 MODEL 1
Complete list - r 2 2 Bytes