Header list of 1lvr.pdb file
Complete list - 27 202 Bytes
HEADER SIGNALING PROTEIN 29-MAY-02 1LVR TITLE IC3 OF CB1 (L431A,A432L) BOUND TO G(ALPHA)I COMPND MOL_ID: 1; COMPND 2 MOLECULE: CANNABINOID RECEPTOR 1; COMPND 3 CHAIN: A; COMPND 4 FRAGMENT: IC3 OF CB1(RESIDUES 338-346); COMPND 5 SYNONYM: CB1, CB-R, CANN6; COMPND 6 ENGINEERED: YES; COMPND 7 MUTATION: YES SOURCE MOL_ID: 1; SOURCE 2 SYNTHETIC: YES; SOURCE 3 OTHER_DETAILS: THE PROTEIN WAS SYNTHESIZED USING SOLID PHASE SOURCE 4 SYNTHESIS. THE SEQUENCE OF THE PROTEIN IS NATURALLY FOUND IN HOMO SOURCE 5 SAPIENS. KEYWDS INTRACELLULAR LOOP 3 (IC3), CANNABINOID 1 RECEPTOR (CB1), ALPHA KEYWDS 2 DOMAIN OF G PROTEIN I, TRANSFERRED NOES, SIGNALING PROTEIN EXPDTA SOLUTION NMR AUTHOR A.L.ULFERS,J.L.MCMURRY,A.MILLER,L.WANG,D.A.KENDALL,D.F.MIERKE REVDAT 3 27-OCT-21 1LVR 1 REMARK SEQADV REVDAT 2 24-FEB-09 1LVR 1 VERSN REVDAT 1 11-DEC-02 1LVR 0 JRNL AUTH A.L.ULFERS,J.L.MCMURRY,A.MILLER,L.WANG,D.A.KENDALL, JRNL AUTH 2 D.F.MIERKE JRNL TITL CANNABINOID RECEPTOR-G PROTEIN INTERACTIONS: JRNL TITL 2 G(ALPHAI1)-BOUND STRUCTURES OF IC3 AND A MUTANT WITH ALTERED JRNL TITL 3 G PROTEIN SPECIFICITY. JRNL REF PROTEIN SCI. V. 11 2526 2002 JRNL REFN ISSN 0961-8368 JRNL PMID 12237474 JRNL DOI 10.1110/PS.0218402 REMARK 2 REMARK 2 RESOLUTION. NOT APPLICABLE. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 PROGRAM : FELIX MSI 95, DGII HOME WRITTEN REMARK 3 AUTHORS : HARE (FELIX), HAVEL (DGII) REMARK 3 REMARK 3 OTHER REFINEMENT REMARKS: NULL REMARK 4 REMARK 4 1LVR COMPLIES WITH FORMAT V. 3.30, 13-JUL-11 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 30-MAY-02. REMARK 100 THE DEPOSITION ID IS D_1000016322. REMARK 210 REMARK 210 EXPERIMENTAL DETAILS REMARK 210 EXPERIMENT TYPE : NMR REMARK 210 TEMPERATURE (KELVIN) : 308 REMARK 210 PH : 6.0 REMARK 210 IONIC STRENGTH : 10 MM ACETATE BUFFER REMARK 210 PRESSURE : 1 ATM REMARK 210 SAMPLE CONTENTS : ALKT 4.0 MM G(ALPHA)I 200 UM REMARK 210 REMARK 210 NMR EXPERIMENTS CONDUCTED : 2D NOESY; 2D TOCSY REMARK 210 SPECTROMETER FIELD STRENGTH : 600 MHZ REMARK 210 SPECTROMETER MODEL : AVANCE REMARK 210 SPECTROMETER MANUFACTURER : BRUKER REMARK 210 REMARK 210 STRUCTURE DETERMINATION. REMARK 210 SOFTWARE USED : IRMA 2 REMARK 210 METHOD USED : METRIC MATRIX DISTANCE GEOMETRY REMARK 210 TO GENERATE INITIAL ENSEMBLE OF REMARK 210 STRUCTURES; 20 LOWEST PENALTY REMARK 210 STRUCTURES USED FOR ENSEMBLE- REMARK 210 BASED IRMA REFINEMENT REMARK 210 REMARK 210 CONFORMERS, NUMBER CALCULATED : 20 REMARK 210 CONFORMERS, NUMBER SUBMITTED : 1 REMARK 210 CONFORMERS, SELECTION CRITERIA : BACK CALCULATED DATA AGREE WITH REMARK 210 EXPERIMENTAL NOESY SPECTRUM REMARK 210 REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL REMARK 210 REMARK 210 REMARK: TRANSFERRED NOES, BUILD UP RATES FROM 6 MIXING TIMES REMARK 215 REMARK 215 NMR STUDY REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON REMARK 215 THESE RECORDS ARE MEANINGLESS. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC REMARK 350 APPLY THE FOLLOWING TO CHAINS: A REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: COVALENT BOND ANGLES REMARK 500 REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1) REMARK 500 REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999 REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996 REMARK 500 REMARK 500 M RES CSSEQI ATM1 ATM2 ATM3 REMARK 500 ARG A 3 NE - CZ - NH1 ANGL. DEV. = 3.7 DEGREES REMARK 500 REMARK 500 REMARK: NULL REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2) REMARK 500 REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI- REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400 REMARK 500 REMARK 500 M RES CSSEQI PSI PHI REMARK 500 ARG A 3 -74.39 -138.05 REMARK 500 ALA A 4 -91.32 -131.33 REMARK 500 THR A 7 -55.92 -121.46 REMARK 500 REMARK 500 REMARK: NULL REMARK 900 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: 1LVQ RELATED DB: PDB REMARK 900 WILD-TYPE IC3 PEPTIDE DBREF 1LVR A 1 9 UNP P21554 CNR1_HUMAN 338 346 SEQADV 1LVR ALA A 4 UNP P21554 LEU 341 ENGINEERED MUTATION SEQADV 1LVR LEU A 5 UNP P21554 ALA 342 ENGINEERED MUTATION SEQRES 1 A 9 ASP ILE ARG ALA LEU LYS THR LEU VAL CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000
Complete list - 27 202 Bytes