Header list of 1irz.pdb file
Complete list - b 23 2 Bytes
HEADER DNA BINDING PROTEIN 25-OCT-01 1IRZ TITLE SOLUTION STRUCTURE OF ARR10-B BELONGING TO THE GARP FAMILY OF PLANT TITLE 2 MYB-RELATED DNA BINDING MOTIFS OF THE ARABIDOPSIS RESPONSE REGULATORS COMPND MOL_ID: 1; COMPND 2 MOLECULE: ARR10-B; COMPND 3 CHAIN: A; COMPND 4 FRAGMENT: DNA BINDING DOMAIN; COMPND 5 SYNONYM: RECEIVER-LIKE PROTEIN 4, ARP4; COMPND 6 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: ARABIDOPSIS THALIANA; SOURCE 3 ORGANISM_COMMON: THALE CRESS; SOURCE 4 ORGANISM_TAXID: 3702; SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3); SOURCE 6 EXPRESSION_SYSTEM_TAXID: 469008; SOURCE 7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3); SOURCE 8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 9 EXPRESSION_SYSTEM_PLASMID: PET22B(+) KEYWDS HELIX-TURN-HELIX, DNA BINDING PROTEIN EXPDTA SOLUTION NMR NUMMDL 15 AUTHOR T.YAMAZAKI,E.KATOH,K.HOSODA,T.MIZUNO REVDAT 3 23-FEB-22 1IRZ 1 REMARK REVDAT 2 24-FEB-09 1IRZ 1 VERSN REVDAT 1 11-FEB-03 1IRZ 0 JRNL AUTH K.HOSODA,A.IMAMURA,E.KATOH,T.HATTA,M.TACHIKI,H.YAMADA, JRNL AUTH 2 T.MIZUNO,T.YAMAZAKI JRNL TITL MOLECULAR STRUCTURE OF THE GARP FAMILY OF PLANT MYB-RELATED JRNL TITL 2 DNA BINDING MOTIFS OF THE ARABIDOPSIS RESPONSE REGULATORS JRNL REF PLANT CELL V. 14 2015 2003 JRNL REFN ISSN 1040-4651 JRNL PMID 12215502 JRNL DOI 10.1105/TPC.002733 REMARK 2 REMARK 2 RESOLUTION. NOT APPLICABLE. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 PROGRAM : NMRPIPE 1.8, X-PLOR 3.1 REMARK 3 AUTHORS : FRANK DELAGLIO (NMRPIPE), BRUNGER (X-PLOR) REMARK 3 REMARK 3 OTHER REFINEMENT REMARKS: NULL REMARK 4 REMARK 4 1IRZ COMPLIES WITH FORMAT V. 3.15, 01-DEC-08 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 01-NOV-01. REMARK 100 THE DEPOSITION ID IS D_1000005220. REMARK 210 REMARK 210 EXPERIMENTAL DETAILS REMARK 210 EXPERIMENT TYPE : NMR REMARK 210 TEMPERATURE (KELVIN) : 288 REMARK 210 PH : 6.9 REMARK 210 IONIC STRENGTH : 500MM NACL REMARK 210 PRESSURE : AMBIENT REMARK 210 SAMPLE CONTENTS : 1MM ARR10-B[U-15N,13C]; 50MM REMARK 210 SODIUM PHOSPHATE BUFFER 500MM REMARK 210 NACL; 1MM ARR10-B[U-15N,13C]; REMARK 210 50MM SODIUM PHOSPHATE BUFFER REMARK 210 500MM NACL REMARK 210 REMARK 210 NMR EXPERIMENTS CONDUCTED : 3D_15N-SEPARATED_NOESY; 4D_13C REMARK 210 -SEPARATED_NOESY; HCCH-TOCSY; REMARK 210 CBCA(CO)NH; HNCO; HNCA REMARK 210 SPECTROMETER FIELD STRENGTH : 750 MHZ REMARK 210 SPECTROMETER MODEL : DMX REMARK 210 SPECTROMETER MANUFACTURER : BRUKER REMARK 210 REMARK 210 STRUCTURE DETERMINATION. REMARK 210 SOFTWARE USED : PIPP/CAPP/STAPP 4.3.2 REMARK 210 METHOD USED : DISTANCE GEOMETRY SIMULATED REMARK 210 ANNEALING REMARK 210 REMARK 210 CONFORMERS, NUMBER CALCULATED : 50 REMARK 210 CONFORMERS, NUMBER SUBMITTED : 15 REMARK 210 CONFORMERS, SELECTION CRITERIA : STRUCTURES WITH LOWEST ENERGIES REMARK 210 REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL REMARK 210 REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED BY MULTIDIMENSIONAL REMARK 210 HETERONUCLEAR NMR SPECTROSCOPY. REMARK 215 REMARK 215 NMR STUDY REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON REMARK 215 THESE RECORDS ARE MEANINGLESS. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC REMARK 350 APPLY THE FOLLOWING TO CHAINS: A REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2) REMARK 500 REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI- REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400 REMARK 500 REMARK 500 M RES CSSEQI PSI PHI REMARK 500 1 ALA A 2 167.06 176.81 REMARK 500 1 GLN A 3 -179.84 -50.06 REMARK 500 1 LYS A 4 55.20 -155.58 REMARK 500 1 ARG A 7 90.45 38.60 REMARK 500 1 TRP A 10 79.74 -160.86 REMARK 500 1 LYS A 43 -2.23 77.19 REMARK 500 1 LYS A 61 -81.16 -61.16 REMARK 500 2 ALA A 2 -178.42 66.18 REMARK 500 2 LYS A 5 98.65 60.88 REMARK 500 2 ARG A 7 87.49 43.58 REMARK 500 2 TRP A 10 76.29 -169.85 REMARK 500 2 LYS A 43 -2.25 77.03 REMARK 500 2 LYS A 62 -33.05 -130.34 REMARK 500 3 GLN A 3 108.98 66.47 REMARK 500 3 LYS A 5 100.67 57.32 REMARK 500 3 TRP A 10 74.95 -164.17 REMARK 500 4 LYS A 5 132.42 61.45 REMARK 500 4 TRP A 10 67.83 -174.10 REMARK 500 5 ALA A 2 111.10 165.87 REMARK 500 5 ARG A 7 90.98 45.22 REMARK 500 5 TRP A 10 63.29 -171.74 REMARK 500 5 LYS A 43 -3.16 78.05 REMARK 500 5 LYS A 62 -48.30 -137.92 REMARK 500 6 ALA A 2 71.36 -119.38 REMARK 500 6 LYS A 4 -173.35 -52.83 REMARK 500 6 ARG A 7 95.02 -43.68 REMARK 500 6 LEU A 9 -60.44 -93.44 REMARK 500 6 TRP A 10 76.13 -150.26 REMARK 500 6 LYS A 43 -8.48 80.22 REMARK 500 6 LYS A 62 59.85 -116.97 REMARK 500 7 GLN A 3 139.49 71.71 REMARK 500 7 LYS A 5 99.37 50.96 REMARK 500 7 ARG A 7 105.75 52.51 REMARK 500 7 TRP A 10 70.39 -170.82 REMARK 500 7 LYS A 43 -7.44 81.84 REMARK 500 8 ALA A 2 -176.39 57.99 REMARK 500 8 LYS A 4 -179.08 -52.72 REMARK 500 8 ARG A 7 100.83 -43.07 REMARK 500 8 TRP A 10 70.02 -168.00 REMARK 500 8 LYS A 43 -0.50 76.37 REMARK 500 9 ALA A 2 60.98 72.31 REMARK 500 9 GLN A 3 55.49 -152.13 REMARK 500 9 LYS A 4 48.20 -178.24 REMARK 500 9 LYS A 5 109.69 56.32 REMARK 500 9 TRP A 10 68.02 -168.97 REMARK 500 9 LYS A 43 -3.76 79.07 REMARK 500 10 ALA A 2 177.83 53.30 REMARK 500 10 GLN A 3 51.94 -114.34 REMARK 500 10 LYS A 5 107.17 -164.48 REMARK 500 10 ARG A 7 103.55 -40.24 REMARK 500 REMARK 500 THIS ENTRY HAS 87 RAMACHANDRAN OUTLIERS. REMARK 500 REMARK 500 REMARK: NULL REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: PLANAR GROUPS REMARK 500 REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS REMARK 500 AN RMSD GREATER THAN THIS VALUE REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 M RES CSSEQI RMS TYPE REMARK 500 1 ARG A 7 0.29 SIDE CHAIN REMARK 500 1 ARG A 29 0.12 SIDE CHAIN REMARK 500 1 ARG A 46 0.23 SIDE CHAIN REMARK 500 1 ARG A 57 0.32 SIDE CHAIN REMARK 500 2 ARG A 7 0.09 SIDE CHAIN REMARK 500 2 ARG A 29 0.31 SIDE CHAIN REMARK 500 2 ARG A 46 0.09 SIDE CHAIN REMARK 500 2 ARG A 57 0.17 SIDE CHAIN REMARK 500 3 ARG A 7 0.26 SIDE CHAIN REMARK 500 3 ARG A 29 0.32 SIDE CHAIN REMARK 500 3 ARG A 46 0.19 SIDE CHAIN REMARK 500 3 ARG A 57 0.16 SIDE CHAIN REMARK 500 4 ARG A 7 0.23 SIDE CHAIN REMARK 500 4 ARG A 29 0.20 SIDE CHAIN REMARK 500 4 ARG A 46 0.18 SIDE CHAIN REMARK 500 4 ARG A 57 0.19 SIDE CHAIN REMARK 500 5 ARG A 7 0.29 SIDE CHAIN REMARK 500 5 ARG A 29 0.19 SIDE CHAIN REMARK 500 5 ARG A 46 0.31 SIDE CHAIN REMARK 500 5 ARG A 57 0.12 SIDE CHAIN REMARK 500 6 ARG A 7 0.18 SIDE CHAIN REMARK 500 6 ARG A 29 0.31 SIDE CHAIN REMARK 500 6 ARG A 46 0.27 SIDE CHAIN REMARK 500 6 ARG A 57 0.21 SIDE CHAIN REMARK 500 7 ARG A 29 0.12 SIDE CHAIN REMARK 500 7 ARG A 57 0.26 SIDE CHAIN REMARK 500 8 ARG A 7 0.22 SIDE CHAIN REMARK 500 8 ARG A 29 0.31 SIDE CHAIN REMARK 500 8 ARG A 46 0.18 SIDE CHAIN REMARK 500 8 ARG A 57 0.31 SIDE CHAIN REMARK 500 9 ARG A 7 0.29 SIDE CHAIN REMARK 500 9 ARG A 29 0.30 SIDE CHAIN REMARK 500 9 ARG A 46 0.30 SIDE CHAIN REMARK 500 9 ARG A 57 0.09 SIDE CHAIN REMARK 500 10 ARG A 7 0.22 SIDE CHAIN REMARK 500 10 ARG A 29 0.15 SIDE CHAIN REMARK 500 10 ARG A 46 0.09 SIDE CHAIN REMARK 500 10 ARG A 57 0.25 SIDE CHAIN REMARK 500 11 ARG A 7 0.32 SIDE CHAIN REMARK 500 11 ARG A 29 0.23 SIDE CHAIN REMARK 500 11 ARG A 57 0.30 SIDE CHAIN REMARK 500 12 ARG A 7 0.15 SIDE CHAIN REMARK 500 12 ARG A 29 0.31 SIDE CHAIN REMARK 500 12 ARG A 46 0.32 SIDE CHAIN REMARK 500 12 ARG A 57 0.32 SIDE CHAIN REMARK 500 13 ARG A 7 0.31 SIDE CHAIN REMARK 500 13 ARG A 29 0.31 SIDE CHAIN REMARK 500 13 ARG A 46 0.22 SIDE CHAIN REMARK 500 13 ARG A 57 0.10 SIDE CHAIN REMARK 500 14 ARG A 7 0.27 SIDE CHAIN REMARK 500 REMARK 500 THIS ENTRY HAS 57 PLANE DEVIATIONS. REMARK 500 REMARK 500 REMARK: NULL REMARK 900 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: 5174 RELATED DB: BMRB REMARK 900 5174 CONTAINS CHEMICAL SHIFTS DBREF 1IRZ A 1 64 UNP O49397 ARR10_ARATH 179 242 SEQRES 1 A 64 THR ALA GLN LYS LYS PRO ARG VAL LEU TRP THR HIS GLU SEQRES 2 A 64 LEU HIS ASN LYS PHE LEU ALA ALA VAL ASP HIS LEU GLY SEQRES 3 A 64 VAL GLU ARG ALA VAL PRO LYS LYS ILE LEU ASP LEU MET SEQRES 4 A 64 ASN VAL ASP LYS LEU THR ARG GLU ASN VAL ALA SER HIS SEQRES 5 A 64 LEU GLN LYS PHE ARG VAL ALA LEU LYS LYS VAL SER HELIX 1 1 THR A 11 GLY A 26 1 16 HELIX 2 2 VAL A 31 ASN A 40 1 10 HELIX 3 3 THR A 45 LYS A 62 1 18 CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000 MODEL 1
Complete list - b 23 2 Bytes