Header list of 1g70.pdb file
Complete list - b 23 2 Bytes
HEADER VIRAL PROTEIN/RNA 08-NOV-00 1G70
TITLE COMPLEX OF HIV-1 RRE-IIB RNA WITH RSG-1.2 PEPTIDE
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: HIV-1 RRE-IIB 32 NUCLEOTIDE RNA;
COMPND 3 CHAIN: A;
COMPND 4 ENGINEERED: YES;
COMPND 5 MOL_ID: 2;
COMPND 6 MOLECULE: RSG-1.2 PEPTIDE;
COMPND 7 CHAIN: B;
COMPND 8 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: HUMAN IMMUNODEFICIENCY VIRUS 1;
SOURCE 3 ORGANISM_TAXID: 11676;
SOURCE 4 OTHER_DETAILS: T7 IN VITRO TRANSCRIPTION;
SOURCE 5 MOL_ID: 2;
SOURCE 6 ORGANISM_SCIENTIFIC: HUMAN IMMUNODEFICIENCY VIRUS 1;
SOURCE 7 ORGANISM_TAXID: 11676;
SOURCE 8 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE 9 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE 10 OTHER_DETAILS: IN VIVO-SELECTED PEPTIDE
KEYWDS PEPTIDE-RNA COMPLEX, NON-CANONICAL BASE PAIRS, VIRAL PROTEIN-RNA
KEYWDS 2 COMPLEX
EXPDTA SOLUTION NMR
NUMMDL 14
AUTHOR Y.GOSSER,T.HERMANN,A.MAJUMDAR,W.HU,R.FREDERICK,F.JIANG,W.XU,D.J.PATEL
REVDAT 3 23-FEB-22 1G70 1 REMARK
REVDAT 2 24-FEB-09 1G70 1 VERSN
REVDAT 1 07-FEB-01 1G70 0
JRNL AUTH Y.GOSSER,T.HERMANN,A.MAJUMDAR,W.HU,R.FREDERICK,F.JIANG,W.XU,
JRNL AUTH 2 D.J.PATEL
JRNL TITL PEPTIDE-TRIGGERED CONFORMATIONAL SWITCH IN HIV-1 RRE RNA
JRNL TITL 2 COMPLEXES.
JRNL REF NAT.STRUCT.BIOL. V. 8 146 2001
JRNL REFN ISSN 1072-8368
JRNL PMID 11175904
JRNL DOI 10.1038/84138
REMARK 1
REMARK 1 REFERENCE 1
REMARK 1 AUTH K.HARADA,S.S.MARTIN,R.TAN,A.D.FRANKEL
REMARK 1 TITL MOLDING A PEPTIDE INTO AN RNA SITE BY IN VIVO PEPTIDE
REMARK 1 TITL 2 EVOLUTION
REMARK 1 REF PROC.NATL.ACAD.SCI.USA V. 94 11887 1997
REMARK 1 REFN ISSN 0027-8424
REMARK 1 DOI 10.1073/PNAS.94.22.11887
REMARK 1 REFERENCE 2
REMARK 1 AUTH J.L.BATTISTE,H.MAO,N.S.RAO,R.TAN,D.R.MUHANDIRAM,L.E.KAY,
REMARK 1 AUTH 2 A.D.FRANKEL,J.R.WILLIAMSON
REMARK 1 TITL ALPHA HELIX-RNA MAJOR GROOVE RECOGNITION IN AN HIV-1 REV
REMARK 1 TITL 2 PEPTIDE-RRE RNA COMPLEX
REMARK 1 REF SCIENCE V. 273 1547 1996
REMARK 1 REFN ISSN 0036-8075
REMARK 2
REMARK 2 RESOLUTION. NOT APPLICABLE.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : NMRPIPE 1, AMBER 4.1
REMARK 3 AUTHORS : DELAGLIO, F., ET AL. (NMRPIPE), KOLLMAN, P., ET
REMARK 3 AL. (AMBER)
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 1G70 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 16-NOV-00.
REMARK 100 THE DEPOSITION ID IS D_1000012312.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210 EXPERIMENT TYPE : NMR
REMARK 210 TEMPERATURE (KELVIN) : 298
REMARK 210 PH : 6.0
REMARK 210 IONIC STRENGTH : 10MM SODIUM PHOSPHATE, 12.5MM
REMARK 210 SODIUM ACETATE, 25MM SODIUM
REMARK 210 CHLORIDE, 0.1MM EDTA
REMARK 210 PRESSURE : AMBIENT
REMARK 210 SAMPLE CONTENTS : NULL
REMARK 210
REMARK 210 NMR EXPERIMENTS CONDUCTED : 2D HOMONUCLEAR EXPERIMENTS, 2D,
REMARK 210 3D HETERONUCLEAR EXPERIMENTS
REMARK 210 SPECTROMETER FIELD STRENGTH : 600 MHZ
REMARK 210 SPECTROMETER MODEL : INOVA
REMARK 210 SPECTROMETER MANUFACTURER : VARIAN
REMARK 210
REMARK 210 STRUCTURE DETERMINATION.
REMARK 210 SOFTWARE USED : NMRVIEW 2.1.2, AMBER 4.1
REMARK 210 METHOD USED : SIMULATED ANNEALING, MOLECULAR
REMARK 210 DYNAMICS
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED : 90
REMARK 210 CONFORMERS, NUMBER SUBMITTED : 14
REMARK 210 CONFORMERS, SELECTION CRITERIA : STRUCTURES WITH ACCEPTABLE
REMARK 210 COVALENT GEOMETRY,STRUCTURES
REMARK 210 WITH THE LEAST RESTRAINT
REMARK 210 VIOLATIONS,STRUCTURES WITH THE
REMARK 210 LOWEST ENERGY
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1
REMARK 210
REMARK 210 REMARK: NULL
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 465 SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465 MODELS 1-14
REMARK 465 RES C SSSEQI
REMARK 465 ASP B 1
REMARK 465 ARG B 2
REMARK 465 ARG B 3
REMARK 465 ARG B 4
REMARK 465 ARG B 5
REMARK 465 GLY B 6
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500 ATM1 RES C SSEQI ATM2 RES C SSEQI DISTANCE
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500 M RES CSSEQI ATM1 ATM2 ATM3
REMARK 500 1 U A 43 O4' - C1' - N1 ANGL. DEV. = 4.5 DEGREES
REMARK 500 1 G A 47 O4' - C1' - N9 ANGL. DEV. = 8.0 DEGREES
REMARK 500 1 C A 49 C5' - C4' - O4' ANGL. DEV. = 6.6 DEGREES
REMARK 500 1 C A 49 O4' - C1' - N1 ANGL. DEV. = 10.1 DEGREES
REMARK 500 1 C A 49 N1 - C2 - O2 ANGL. DEV. = 3.6 DEGREES
REMARK 500 1 G A 50 O4' - C1' - N9 ANGL. DEV. = 5.0 DEGREES
REMARK 500 1 C A 51 C5' - C4' - C3' ANGL. DEV. = -8.5 DEGREES
REMARK 500 1 C A 62 O4' - C1' - N1 ANGL. DEV. = 6.9 DEGREES
REMARK 500 1 U A 66 C3' - O3' - P ANGL. DEV. = 7.6 DEGREES
REMARK 500 1 A A 68 O4' - C1' - N9 ANGL. DEV. = 5.9 DEGREES
REMARK 500 1 U A 72 C3' - O3' - P ANGL. DEV. = 8.5 DEGREES
REMARK 500 1 C A 74 O4' - C1' - N1 ANGL. DEV. = 4.7 DEGREES
REMARK 500 2 C A 44 O4' - C1' - N1 ANGL. DEV. = 4.2 DEGREES
REMARK 500 2 U A 45 O5' - C5' - C4' ANGL. DEV. = -5.0 DEGREES
REMARK 500 2 G A 48 C5' - C4' - O4' ANGL. DEV. = 5.7 DEGREES
REMARK 500 2 G A 48 O4' - C1' - N9 ANGL. DEV. = 7.7 DEGREES
REMARK 500 2 C A 51 O4' - C1' - N1 ANGL. DEV. = 4.3 DEGREES
REMARK 500 2 U A 60 O4' - C1' - N1 ANGL. DEV. = 6.0 DEGREES
REMARK 500 2 C A 62 O4' - C1' - N1 ANGL. DEV. = 5.8 DEGREES
REMARK 500 2 U A 66 O4' - C1' - N1 ANGL. DEV. = 5.0 DEGREES
REMARK 500 2 A A 68 N9 - C1' - C2' ANGL. DEV. = -7.6 DEGREES
REMARK 500 2 A A 68 O4' - C1' - N9 ANGL. DEV. = 7.8 DEGREES
REMARK 500 2 A A 68 C3' - O3' - P ANGL. DEV. = 7.9 DEGREES
REMARK 500 2 C A 69 C3' - O3' - P ANGL. DEV. = 8.7 DEGREES
REMARK 500 2 U A 72 C5' - C4' - O4' ANGL. DEV. = -9.8 DEGREES
REMARK 500 2 U A 72 C3' - O3' - P ANGL. DEV. = 8.1 DEGREES
REMARK 500 2 ARG B 14 NE - CZ - NH2 ANGL. DEV. = -3.6 DEGREES
REMARK 500 3 U A 43 O4' - C1' - N1 ANGL. DEV. = 4.5 DEGREES
REMARK 500 3 U A 45 O4' - C1' - N1 ANGL. DEV. = 5.5 DEGREES
REMARK 500 3 G A 47 O4' - C1' - N9 ANGL. DEV. = 7.0 DEGREES
REMARK 500 3 G A 48 O4' - C1' - N9 ANGL. DEV. = 6.3 DEGREES
REMARK 500 3 C A 49 O4' - C1' - N1 ANGL. DEV. = 8.4 DEGREES
REMARK 500 3 U A 60 O4' - C1' - N1 ANGL. DEV. = 4.8 DEGREES
REMARK 500 3 G A 63 N3 - C4 - C5 ANGL. DEV. = -3.1 DEGREES
REMARK 500 3 C A 69 O4' - C1' - N1 ANGL. DEV. = 9.7 DEGREES
REMARK 500 3 U A 72 C1' - O4' - C4' ANGL. DEV. = -4.6 DEGREES
REMARK 500 3 U A 72 C3' - O3' - P ANGL. DEV. = 7.4 DEGREES
REMARK 500 3 G A 76 O4' - C1' - N9 ANGL. DEV. = 4.4 DEGREES
REMARK 500 4 C A 44 O4' - C1' - N1 ANGL. DEV. = 4.4 DEGREES
REMARK 500 4 U A 45 N1 - C1' - C2' ANGL. DEV. = 9.2 DEGREES
REMARK 500 4 C A 49 O4' - C1' - N1 ANGL. DEV. = 5.4 DEGREES
REMARK 500 4 C A 49 N1 - C2 - O2 ANGL. DEV. = 3.9 DEGREES
REMARK 500 4 C A 62 O4' - C1' - N1 ANGL. DEV. = 6.2 DEGREES
REMARK 500 4 G A 67 N9 - C1' - C2' ANGL. DEV. = -7.0 DEGREES
REMARK 500 4 U A 72 C5' - C4' - O4' ANGL. DEV. = -8.4 DEGREES
REMARK 500 4 U A 72 O4' - C1' - N1 ANGL. DEV. = 5.9 DEGREES
REMARK 500 4 U A 72 C3' - O3' - P ANGL. DEV. = 8.6 DEGREES
REMARK 500 4 A A 73 O4' - C1' - N9 ANGL. DEV. = 5.5 DEGREES
REMARK 500 5 U A 43 O4' - C1' - N1 ANGL. DEV. = 5.2 DEGREES
REMARK 500 5 C A 44 O4' - C1' - N1 ANGL. DEV. = 4.4 DEGREES
REMARK 500
REMARK 500 THIS ENTRY HAS 216 ANGLE DEVIATIONS.
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 1 SER B 10 21.07 -67.64
REMARK 500 2 SER B 10 17.74 -60.08
REMARK 500 3 ALA B 21 -78.22 -103.54
REMARK 500 4 SER B 10 8.55 -49.50
REMARK 500 5 SER B 10 5.51 -63.91
REMARK 500 7 SER B 10 14.33 -59.02
REMARK 500 8 SER B 10 2.43 -67.51
REMARK 500 9 PRO B 9 138.11 -36.84
REMARK 500 9 SER B 10 -1.29 -55.36
REMARK 500 12 PRO B 9 107.81 -50.77
REMARK 500 12 SER B 10 30.72 -72.60
REMARK 500 14 SER B 10 15.95 -64.50
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: PLANAR GROUPS
REMARK 500
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS
REMARK 500 AN RMSD GREATER THAN THIS VALUE
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 M RES CSSEQI RMS TYPE
REMARK 500 1 C A 44 0.16 SIDE CHAIN
REMARK 500 1 G A 46 0.07 SIDE CHAIN
REMARK 500 1 G A 47 0.10 SIDE CHAIN
REMARK 500 1 G A 48 0.07 SIDE CHAIN
REMARK 500 1 G A 50 0.09 SIDE CHAIN
REMARK 500 1 G A 64 0.13 SIDE CHAIN
REMARK 500 1 U A 66 0.07 SIDE CHAIN
REMARK 500 1 G A 67 0.06 SIDE CHAIN
REMARK 500 1 A A 68 0.11 SIDE CHAIN
REMARK 500 1 G A 70 0.09 SIDE CHAIN
REMARK 500 1 C A 74 0.11 SIDE CHAIN
REMARK 500 1 G A 76 0.07 SIDE CHAIN
REMARK 500 1 C A 78 0.18 SIDE CHAIN
REMARK 500 1 C A 79 0.16 SIDE CHAIN
REMARK 500 1 ARG B 16 0.09 SIDE CHAIN
REMARK 500 2 G A 41 0.07 SIDE CHAIN
REMARK 500 2 G A 42 0.07 SIDE CHAIN
REMARK 500 2 G A 46 0.07 SIDE CHAIN
REMARK 500 2 G A 47 0.12 SIDE CHAIN
REMARK 500 2 G A 48 0.08 SIDE CHAIN
REMARK 500 2 C A 49 0.09 SIDE CHAIN
REMARK 500 2 C A 51 0.10 SIDE CHAIN
REMARK 500 2 U A 60 0.08 SIDE CHAIN
REMARK 500 2 U A 61 0.06 SIDE CHAIN
REMARK 500 2 G A 64 0.12 SIDE CHAIN
REMARK 500 2 A A 68 0.09 SIDE CHAIN
REMARK 500 2 C A 69 0.13 SIDE CHAIN
REMARK 500 2 G A 70 0.07 SIDE CHAIN
REMARK 500 2 U A 72 0.14 SIDE CHAIN
REMARK 500 2 C A 74 0.19 SIDE CHAIN
REMARK 500 2 C A 78 0.10 SIDE CHAIN
REMARK 500 2 C A 79 0.15 SIDE CHAIN
REMARK 500 3 G A 41 0.06 SIDE CHAIN
REMARK 500 3 C A 44 0.09 SIDE CHAIN
REMARK 500 3 G A 47 0.09 SIDE CHAIN
REMARK 500 3 G A 48 0.09 SIDE CHAIN
REMARK 500 3 G A 63 0.12 SIDE CHAIN
REMARK 500 3 G A 64 0.15 SIDE CHAIN
REMARK 500 3 A A 68 0.12 SIDE CHAIN
REMARK 500 3 G A 70 0.07 SIDE CHAIN
REMARK 500 3 G A 71 0.09 SIDE CHAIN
REMARK 500 3 U A 72 0.09 SIDE CHAIN
REMARK 500 3 C A 74 0.10 SIDE CHAIN
REMARK 500 3 A A 75 0.09 SIDE CHAIN
REMARK 500 3 C A 78 0.09 SIDE CHAIN
REMARK 500 3 C A 79 0.08 SIDE CHAIN
REMARK 500 3 ARG B 17 0.10 SIDE CHAIN
REMARK 500 4 G A 42 0.10 SIDE CHAIN
REMARK 500 4 G A 46 0.06 SIDE CHAIN
REMARK 500 4 G A 47 0.10 SIDE CHAIN
REMARK 500
REMARK 500 THIS ENTRY HAS 216 PLANE DEVIATIONS.
REMARK 500
REMARK 500 REMARK: NULL
DBREF 1G70 B 1 22 UNP Q7SIF5 Q7SIF5_9HIV1 1 22
DBREF 1G70 A 41 79 PDB 1G70 1G70 41 79
SEQRES 1 A 32 G G U C U G G G C G C A C
SEQRES 2 A 32 U U C G G U G A C G G U A
SEQRES 3 A 32 C A G G C C
SEQRES 1 B 22 ASP ARG ARG ARG ARG GLY SER ARG PRO SER GLY ALA GLU
SEQRES 2 B 22 ARG ARG ARG ARG ARG ALA ALA ALA ALA
HELIX 1 1 GLY B 11 ALA B 22 1 12
CISPEP 1 SER B 7 ARG B 8 1 1.63
CISPEP 2 SER B 7 ARG B 8 2 9.33
CISPEP 3 SER B 7 ARG B 8 3 -3.80
CISPEP 4 SER B 7 ARG B 8 4 1.88
CISPEP 5 SER B 7 ARG B 8 5 17.07
CISPEP 6 SER B 7 ARG B 8 6 2.52
CISPEP 7 SER B 7 ARG B 8 7 -3.07
CISPEP 8 SER B 7 ARG B 8 8 19.85
CISPEP 9 SER B 7 ARG B 8 9 10.88
CISPEP 10 SER B 7 ARG B 8 10 16.30
CISPEP 11 SER B 7 ARG B 8 11 -7.31
CISPEP 12 SER B 7 ARG B 8 12 -22.15
CISPEP 13 SER B 7 ARG B 8 13 0.50
CISPEP 14 SER B 7 ARG B 8 14 -3.79
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
MODEL 1
Complete list - b 23 2 Bytes